Selection of Endogenous Control Reference Genes for Studies on Type 1 or Type 2 Endometrial Cancer

Validation of endogenous control reference genes for normalizing gene expression studies in endometrial carcinoma.

Real-time quantitative RT-PCR (qRT-PCR) is a powerful technique used for the relative quantification of target genes, using reference (housekeeping) genes for normalization to ensure the generation of accurate and robust data. A systematic examination of the suitability of endogenous reference genes for gene expression studies in endometrial cancer tissues is absent. The aims of this study were...

Selection of suitable reference genes for real-time PCR studies of early developmental stages of sturgeons

In quantitative real-time PCR, the mRNA level can be quantified in relative terms based on the expression ratio of mRNAs of the target gene and an internal reference gene. Since, an internal standard should be expressed at a constant level among different tissues of an organism at all stages of development, and should be unaffected by the experimental treatment, the stability of different refer...

Ovarian Metastasis in Endometriod Type Endometrial Cancer

Is the positivity of estrogen receptor or progesterone receptor different between type 1 and type 2 endometrial cancer?

Endometrial cancer is a major cancer in women and traditionally divided into type 1 and type 2. It is well known that type 2 endometrial cancer has a poor prognosis. Studies have suggested that estrogen receptor (ER) or progesterone receptor (PR) positive are positively associated with endometrial cancer survive. However whether the positivity of ER or PR is different between cancer types has n...

Type-1 and Type-2 Fuzzy Logic Control Algorithms for Semi-Active Seismic Vibration Control of the College Urban Bridge Using MR Dampers

In this study, the application of type-1 and type-2 fuzzy inference system (FIS) in semi-active seismic vibration control of the College Bridge using magnetorheological (MR) dampers is investigated. For this purpose, a detailed 3D finite element model of the bridge fitted with MR dampers is created in OpenSees. The command voltage of MR dampers is determined by employing both types of FISs in M...